簡(jiǎn)要描述:Illumina/Nextera® XT Index Kit (96 indexes, 384 samples)/FC-131-1002/1 Ea上海易匯生物科技有限公司于17年正式銷售Illumina公司基因芯片、二代測(cè)序NGS測(cè)序儀及相關(guān)測(cè)序試劑盒等產(chǎn)品Illumina北京銷售公司 簇生成和測(cè)序試劑
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品牌 | illumina/美國(guó)因美納 | 貨號(hào) | Illumina FC-131-1002/1 Ea |
---|---|---|---|
規(guī)格 | 1 kit | 供貨周期 | 兩周 |
主要用途 | 小型全基因組測(cè)序 組合試劑 試劑盒 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥 |
Illumina北京銷售公司 簇生成和測(cè)序試劑
Illumina公司,致力于新一代測(cè)序和芯片技術(shù)的生產(chǎn)與開發(fā),提供產(chǎn)品與應(yīng)用資訊
Illumina公司創(chuàng)立于1998年4月,是遺傳變異和生物學(xué)功能分析領(lǐng)域的優(yōu)秀的產(chǎn)品、技術(shù)和服務(wù)供應(yīng)商。通過(guò)幫助客戶加快實(shí)現(xiàn)生物信息的采集、分析和應(yīng)用,來(lái)改善人類健康。
Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products
上海易匯生物科技有限公司于17年正式銷售Illumina公司基因芯片、二代測(cè)序NGS測(cè)序儀及相關(guān)測(cè)序試劑盒等產(chǎn)品
咨詢illumina MiSeq測(cè)序試劑盒等產(chǎn)品歡迎您致電 上海易匯生物科技有限公司:1850 1609 238張經(jīng)理
"Illumina/Nextera® XT Index Kit (96 indexes, 384 samples)/FC-131-1002/1 Ea
" Illumina 產(chǎn)品編號(hào): FC-131-1002美 元 價(jià): $999.00會(huì) 員 價(jià): 待定品 牌: Illumina產(chǎn) 地: 美國(guó)公 司: Illumina, Inc.產(chǎn)品分類: 分子類>二代測(cè)序>DNA文庫(kù)制備試劑盒公司分類: Library Preparation Kits "Product Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.
Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.
Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit
Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots"
上海易匯生物科技有限公司正式銷售illumina 產(chǎn)品 中國(guó)總銷售上海易匯生物科技有限公司,illumina其前身是成立于1990年的專業(yè)從事動(dòng)物血清生產(chǎn)及銷售的生物技術(shù)公司。公司經(jīng)過(guò)多年發(fā)展,穩(wěn)健成長(zhǎng)。目前主要銷售品牌有epicentre,Lucigen,ImmunoReagents,ICLLAB,illumina等試劑盒。
這款試劑盒提供較快較簡(jiǎn)單的操作流程,90分鐘就可以構(gòu)建好上及測(cè)序的文庫(kù),手動(dòng)操作的時(shí)間少于15分鐘。一步即可完成片段打碎和連接接頭的步驟,不需要特殊設(shè)備,實(shí)驗(yàn)室的常規(guī)設(shè)備即可完成整個(gè)文庫(kù)構(gòu)建。并且建好的文庫(kù)可以適用于illumina的任何測(cè)序平臺(tái)。
采用Nextera技術(shù),一步即可完成片段打碎和連接接頭的步驟,不需要特殊設(shè)備,實(shí)驗(yàn)室的常規(guī)設(shè)備即可完成整個(gè)文庫(kù)構(gòu)建。較適合樣本量極其稀少的珍貴樣本進(jìn)行建庫(kù),這種建庫(kù)方法只需要50ng的建庫(kù)起始量。并且可以在任何illumina測(cè)序設(shè)備上進(jìn)行測(cè)序。
Nextera建庫(kù)試劑盒還需要配合使用TruSeq Dual Index Sequencing Primer Box(FC-121-1003單端/PE-121-1003雙端),不論是單端測(cè)序還是雙端測(cè)序,不論是否有index,還是單端或雙端index,這個(gè)試劑盒都是必須的。
· 另外 Nextera Index kit (FC-121-1011 or FC-121-1012)對(duì)于Nextera文庫(kù)構(gòu)建也是必需的。不論是混合多少樣本再一個(gè)pooling中。
illumina TruSeq PCR-Free DNA建庫(kù)試劑盒
illumina FC-121-3001/FC-121-3002/FC-121-3003
為全基因組測(cè)序提供簡(jiǎn)單,易用的樣本制備方案。從小基因組如細(xì)菌基因組到人類基因組的測(cè)序,這款試劑盒都可以從容應(yīng)對(duì)。這個(gè)試劑盒提供了簡(jiǎn)化了的gel-free流程,這對(duì)于測(cè)序具有挑戰(zhàn)性的銷售,或者鑒別變異特別豐富的銷售,以及提高SNP檢出的質(zhì)量都是有好處的。試劑盒包括試劑,樣本純化的beads和index,并且有兩種不同規(guī)格的試劑盒可供選擇。
TruSeq DNA PCR-Free Sample Preparation Kit針對(duì)于高GC含量銷售,啟動(dòng)子銷售和重復(fù)序列具有非常好的覆蓋度,和傳統(tǒng)的試劑盒比起來(lái)具有明顯的進(jìn)步。這個(gè)試劑盒的靈活性還體現(xiàn)在可以根據(jù)實(shí)驗(yàn)的不同需要,選擇不同長(zhǎng)度的回收片段進(jìn)行建庫(kù)。從而達(dá)到符合不同的實(shí)驗(yàn)?zāi)康牡男枨蟆?/span>
PCR-free的意義在于減少文庫(kù)的bias和gaps。結(jié)果就是的數(shù)據(jù)質(zhì)量。這就使得研究者可以準(zhǔn)確的檢測(cè)變異,并且數(shù)量可觀且可靠。的基因組覆蓋度意味著你的結(jié)果具有非常低且非常小的gaps,另外增強(qiáng)了對(duì)高CG/AT銷售的覆蓋。
工業(yè)客戶廣泛采用的DNA樣本制備流程現(xiàn)在得到了加強(qiáng),去除了PCR的過(guò)程,試驗(yàn)速度變得更快,結(jié)果更好?;诖胖榈钠位厥蘸?jiǎn)化了流程。和illumina測(cè)序系統(tǒng)相配合,PCR-free建庫(kù)試劑盒為工業(yè)客戶優(yōu)化流程提供了一系列的便利。
TruSeq? Nano DNA LT Sample Prep Kit
illumina FC-121-4001/4002/4003
illumina FC-121-4001/FC-121-4002/FC-121-4003
能夠高效的利用有限的DNA樣本?;诠I(yè)上廣泛適用的樣本制備流程,低上樣量的方法表現(xiàn)出高覆蓋度,并且減少了bias的引入。這種方法幾乎可以做任何測(cè)序應(yīng)用。
· 專為低樣本量設(shè)計(jì)
· 高覆蓋度
· 快速的樣本制備
兩種試劑盒類型,適用于不同的研究領(lǐng)域,the TruSeq Nano DNA LT Sample Preparation Kit為較少樣本數(shù)量設(shè)計(jì)的,the TruSeq Nano DNA HT Sample Preparation Kit為樣本數(shù)量較多的研究設(shè)計(jì)的。
利用有限的DNA樣本
這款試劑盒低的建庫(kù)起始量為100ng,不需要像以前一樣需要mg級(jí)別的上樣量。這使得研究者可以利用有限的DNA樣本如:癌癥樣本,并且可以保護(hù)樣本用于將來(lái)的實(shí)驗(yàn)和研究。另外提速的試驗(yàn)流程,基于磁珠的片段回收,也同樣減少了實(shí)驗(yàn)操作中樣本的損失。
減少了文庫(kù)中的bias和基因組覆蓋的gaps
TruSeq Nano DNA kits減少了在PCR過(guò)程中,由于擴(kuò)增不均勻?qū)е碌膅aps,以及gaps的數(shù)量。加強(qiáng)了的流程也同樣減少了文庫(kù)中的bias數(shù)目,提高了覆蓋的均一度。
這些試劑盒同樣提供了優(yōu)秀的覆蓋度,對(duì)于GC-rich銷售,啟動(dòng)子銷售,和重復(fù)序列,都進(jìn)行了很好的優(yōu)化。這使得研究者可以獲得更多,更有效的數(shù)據(jù)。
樣本制備少于一天時(shí)間
TruSeq DNA sample制備流程已經(jīng)得到驗(yàn)證,基于磁珠的片段回收已經(jīng)*替代了基于膠回收的制備流程,這使得研究者可以在一天以內(nèi)就可以建庫(kù)完成高質(zhì)量的文庫(kù)。優(yōu)化了的各種不同的讀長(zhǎng),從2× 101 bp 到 2 × 151 bp,TruSeq Nano DNAkit可以滿足任何測(cè)序長(zhǎng)度。Master-mixed試劑,提供了樣本純化的beads,用于純化和片段回收。豐富的TruSeq indices,和優(yōu)化了的流程使得操作更加簡(jiǎn)單,更少的手動(dòng)操作,更少的純化步驟,都讓建庫(kù)變得簡(jiǎn)單。對(duì)于應(yīng)對(duì)大量樣本,也不會(huì)覺(jué)得工作量巨大了。
Nextera XT DNA Sample Preparation Kit
illumina FC-131
這個(gè)試劑盒適合于構(gòu)建小基因組(如:細(xì)菌、古細(xì)菌和病毒)以及擴(kuò)增子測(cè)序,質(zhì)粒測(cè)序等。整個(gè)試驗(yàn)流程不到90分鐘,手動(dòng)操作時(shí)間僅15分鐘,實(shí)驗(yàn)操作特別簡(jiǎn)單。
應(yīng)用Nextera技術(shù),DNA的打斷和加接頭過(guò)程可以在一個(gè)管子里面一步完成,大大節(jié)省了試驗(yàn)時(shí)間。
· Nextera XT支持超低的建庫(kù)起始量,1ng的DNA就可以進(jìn)行建庫(kù),并且對(duì)物種沒(méi)有選擇性,大部分物種都可以采用這種方法。
· Nextera XT技術(shù)還包含了基于磁珠技術(shù)的樣本均一化,這樣就簡(jiǎn)化了在測(cè)序之前混合樣本的時(shí)候需要的文庫(kù)定量過(guò)程。
· Nextera XT文庫(kù)可以適用于illumina的所有測(cè)序平臺(tái)。
Nextera Rapid Capture Exome Kit
illumina FC-140-1001/1002/1003/1004/1005/1006
這個(gè)試劑盒是一個(gè)集成度非常高,它將構(gòu)建文庫(kù)和捕獲過(guò)程整合到一起,這比其他公司的捕獲試劑盒節(jié)省了70%的時(shí)間。
外顯子捕獲試劑盒通過(guò)巧妙地優(yōu)化,選擇了37Mb外顯子銷售,并且只需要測(cè)4Gb的數(shù)據(jù)就可以了。
Expanded Exome Kits 增加了UTRs和miRNA銷售。
識(shí)別編碼變異(coding variants)別其他的方法快70%
Nextera Rapid Capture Exome可以讓研究者大限度的提高實(shí)驗(yàn)室人員的工作效率和測(cè)序儀的使用率,可以在1.5天的時(shí)間內(nèi)同時(shí)完成96個(gè)樣本的捕獲加建庫(kù)。采用HiSeq2500的快速模式,可以在2.5天內(nèi)完成從樣本到得到數(shù)據(jù)的全過(guò)程,相比較其他技術(shù),Nextera技術(shù)相當(dāng)?shù)目?,并且可以保證數(shù)據(jù)的*性和可重復(fù)性。
Nextera Rapid Capture Expanded Exome 對(duì)于既想研究編碼區(qū)又想研究調(diào)控銷售的老師,這款試劑盒是個(gè)非常好的選擇。GWAS研究指出大于80%的疾病相關(guān)變異在編碼區(qū)以外。分析這些銷售可以使研究者發(fā)現(xiàn)影響基因功能的變異,并且這種方法相對(duì)于全基因組重測(cè)序來(lái)說(shuō)也是經(jīng)濟(jì)劃算的。
Nextera Rapid Capture Expanded Exome提供了62Mb銷售,包括外顯子銷售,UTRs和miRNA。這個(gè)試劑盒的特點(diǎn)就是經(jīng)過(guò)非常好的優(yōu)化后,對(duì)于外顯子銷售有很高的覆蓋度。
關(guān)鍵字: illumina總銷售;illumina試劑盒總銷售,illumina中國(guó)總銷售;illumina 總銷售;illumina北京總銷售;
illumina FC-110-3001 PhiX Control v3
illumina FC-110-3002 NextSeq? PhiX Control Kit
illumina FC-121-0202 TruSight Cancer
illumina FC-121-0205 TruSight Inherited Disease Sequencing Panel (48 samples)
illumina FC-121-1003 TruSeq Dual Index Sequencing Primer Box, Single-Read
illumina FC-121-1011 Nextera Index Kit (24 indexes, 96 samples)
illumina FC-121-1012 Nextera Index Kit (96 indexes, 384 samples)
illumina FC-121-1030 Nextera DNA Library Prep Kit (24 samples)
illumina FC-121-1031 Nextera DNA Library Prep Kit (96 samples)
illumina FC-121-3001 TruSeq DNA PCR-Free LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)
illumina FC-121-3002 TruSeq DNA PCR-Free LT Library Preparation Kit - Set B (12 Set B index tubes, 24 samples)
illumina FC-121-3003 TruSeq DNA PCR-Free HT Library Preparation Kit (96 indexes in plate format, 96 samples)
illumina FC-121-4001 TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)
illumina FC-121-4002 TruSeq Nano DNA LT Library Preparation Kit - Set B (12 Set B index tubes, 24 samples)
illumina FC-121-4003 TruSeq Nano DNA HT Library Preparation Kit (96 indexes in plate format, 96 samples)
illumina FC-121-9999 TruSeq FFPE DNA Library Prep QC Kit (24 samples)
illumina FC-123-1203 TruSight? Enrichment Kit (24 Indices - 48 Samples)
illumina FC-123-1205 TruSight? Enrichment Kit (24 Indices - 96 Samples)
illumina FC-126-1001 TruSeq Synthetic Long-Read DNA Library Prep Kit (4 samples)
illumina FC-126-1002 TruSeq Synthetic Long-Read DNA Barcode Kit (1 barcode plate)
illumina FC-126-1003 TruSeq Synthetic Long-Read DNA Barcode Kit (4 barcode plates)
illumina FC-126-1004 TruSeq Synthetic Long-Read DNA Accessory Kit
illumina FC-130-1003 TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)
illumina FC-130-1005 TruSeq Index Plate Fixture Kit (2 fixtures)
illumina FC-130-1006 TruSeq Custom Amplicon Filter Plate (1 plate)
illumina FC-130-1007 TruSeq Index Plate Fixture & Collar Kit (2 each)
illumina FC-130-1008 TruSeq Amplicon - Cancer Panel (96 samples)
illumina FC-130-1010 TruSight Myeloid Sequencing Panel (96 samples)
illumina FC-130-2001 TruSight Tumor 26
illumina FC-131-1001 Nextera XT Index Kit (24 indexes, 96 samples)
illumina FC-131-1002 Nextera XT Index Kit (96 indexes, 384 samples)
illumina FC-131-1024 Nextera XT DNA Library Preparation Kit (24 samples)
illumina FC-131-1096 Nextera XT DNA Library Preparation Kit (96 samples)
illumina FC-131-2001 Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)
illumina FC-131-2002 Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)
illumina FC-131-2003 Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)
illumina FC-131-2004 Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)
illumina FC-132-1001 Nextera Mate Pair Library Prep Kit (12 indexes, 48 gel-free samples or 12 gel-plus samples)
illumina FC-140-1000 Nextera Rapid Capture Exome (8 rxn x 1 plex)
illumina FC-140-1001 Nextera Rapid Capture Exome (2 rxn x 12 plex)
illumina FC-140-1002 Nextera Rapid Capture Exome (4 rxn x 12 plex)
illumina FC-140-1003 Nextera Rapid Capture Exome (8 rxn x 12 plex)
illumina FC-140-1004 Nextera Rapid Capture Expanded Exome (2 rxn x 12 plex)
illumina FC-140-1005 Nextera Rapid Capture Expanded Exome (4 rxn x 12 plex)
illumina FC-140-1006 Nextera Rapid Capture Expanded Exome (8 rxn x 12 plex)
illumina FC-140-1083 Nextera Rapid Capture Exome (8 rxn x 3 plex)
illumina FC-140-1086 Nextera Rapid Capture Exome (8 rxn x 6 plex)
illumina FC-140-1089 Nextera Rapid Capture Exome (8 rxn x 9 plex)
illumina FC-140-1101 TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)
illumina FC-140-1102 TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)
illumina FC-140-1103 TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)
illumina FC-140-1104 TruSight Rapid Capture(24 indexes, 48 samples, 4 enrichments)
illumina FC-140-1105 TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)
illumina FC-140-1106 TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)
illumina FC-141-1006 TruSight One Sequencing Kit (9 samples)
illumina FC-141-1007 TruSight One Sequencing Panel (36 samples)
illumina FC-141-1010 TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)
illumina FC-141-1011 TruSight Cardio Sequencing Kit for NextSeq (48 indexes, 48 samples, 4 enrichments)
illumina FC-141-2007 TruSight One Expanded Sequencing Panel (36 samples)
illumina FC-142-1001 TruSight? HLA Sequencing Panel (24 samples)
illumina FC-144-1000 TruSeq Rapid Exome Library Prep Kit (8 samples)
illumina FC-144-1001 TruSeq Rapid Exome Library Prep Kit (24 samples)
illumina FC-144-1002 TruSeq Rapid Exome Library Prep Kit (48 samples)
illumina FC-144-1003 TruSeq Rapid Exome Library Prep Kit (72 samples)
illumina FC-144-1004 TruSeq Rapid Exome Library Prep Kit (96 samples)
illumina FC-150-1001 TruSeq Exome Library Prep Kit (24 samples)
illumina FC-150-1002 TruSeq Exome Library Prep Kit (48 samples)
illumina FC-150-1003 TruSeq Exome Library Prep Kit (72 samples)
illumina FC-150-1004 TruSeq Exome Library Prep Kit (96 samples)
illumina FC-151-1002 TruSeq-Methyl Capture EPIC Library Prep Kit (12 samples, 4 indexes)
illumina FC-151-1003 TruSeq-Methyl Capture EPIC Library Prep Kit (48 samples, 12 indexes)
illumina FC-401-3001 TruSeq SBS Kit v3-HS (200 cycles)
illumina FC-401-3002 TruSeq SBS Kit v3-HS (50 cycles)
illumina FC-401-4002 HiSeq SBS Kit V4 50 cycle kit
illumina FC-401-4003 HiSeq SBS Kit V4 250 cycle kit
illumina FC-402-4021 HiSeq Rapid SBS Kit v2 (200 cycles)
illumina FC-402-4022 HiSeq Rapid SBS Kit v2 (50 cycles)
illumina FC-402-4023 HiSeq Rapid SBS Kit v2 (500 cycles)
illumina FC-404-2001 NextSeq 500/550 Mid Output v2 kit (150 cycles)
illumina FC-404-2002 NextSeq 500/550 High Output v2 kit (150 cycles)
illumina FC-404-2003 NextSeq 500/550 Mid Output v2 kit (300 cycles)
illumina FC-404-2004 NextSeq 500/550 High Output v2 kit (300 cycles)
illumina FC-404-2005 NextSeq 500/550 High Output v2 kit (75 cycles)
illumina FC-410-1001 HiSeq 3000/4000 SBS Kit (50 cycles)
illumina FC-410-1002 HiSeq 3000/4000 SBS Kit (150 cycles)
Illumina北京銷售公司 簇生成和測(cè)序試劑
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